Andrea Idili, Alexis Vallée-Bélisle and Francesco Ricci have developed a DNA-based nanosensor that allows to measure pH variation at the nanoscale. This nanosensor may significantly aid efforts to build nanodevices for cancer in-vivo imaging and targeted drug-delivery. This nanosensor measures less than 10 nm and unfolds at a specifically programmed pH. Credit: Marco Tripodi
Bioengineers at the University of Rome Tor Vergata and the University of Montreal have used DNA to develop a tool that detects and reacts to chemical changes caused by cancer cells and that may one day be used to deliver drugs to tumor cells.
The researchers' nanosensor measures pH variations at the nanoscale – how acidic (a higher pH level) or alkaline (a lower pH level) it is. Many biomolecules, such as enzymes and proteins, are strongly regulated by small pH changes. These changes affect in turn biological activities such as enzyme catalysis, protein assembly, membrane function and cell death. There is also a strong relation between cancer and pH.
Cancer cells often display a lower pH compared to normal cells: the pH level inside cancer cells is higher than it is outside. "In living organisms, these small pH changes typically occur in tiny areas measuring only few hundred nanometers," says senior author Prof. Francesco Ricci. "Developing sensors or nanomachines that can measure pH changes at this scale should prove of utility for several applications in the fields of in-vivo imaging, clinical diagnostics and drug-delivery."
"DNA represents an ideal material to build sensors or nanomachines at the nanometer scale" says senior author Prof. Vallée-Bélisle. "By taking advantage of a specific DNA sequences that form pH-sensitive triple helix, we have designed a versatile nanosensor that can be programmed to fluoresce only at specific pH values." Fluorescence is the emission of radiation, including visible light, caused by an exchange of energy. "This programming ability represents a key feature for clinical applications – we can design a specific sensor to send a fluorescent signal only when the pH reaches a specific value which is, for example, characteristic of a specific disease," adds first author Andrea Idili.
In the future, this recently patented nanotechnology may also find applications in the development of novel drug-delivery platforms that release chemio-therapeutic drugs only in the viscinity of tumor cells.
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Andrea Idili, Alexis Vallée-Bélisle and Francesco Ricci have developed a DNA-based nanosensor that allows to measure pH variation at the nanoscale. This nanosensor may significantly aid efforts to build nanodevices for cancer in-vivo imaging and targeted drug-delivery. This nanosensor measures less than 10 nm and unfolds at a specifically programmed pH. Credit: Marco Tripodi
Bioengineers at the University of Rome Tor Vergata and the University of Montreal have used DNA to develop a tool that detects and reacts to chemical changes caused by cancer cells and that may one day be used to deliver drugs to tumor cells.
The researchers' nanosensor measures pH variations at the nanoscale – how acidic (a higher pH level) or alkaline (a lower pH level) it is. Many biomolecules, such as enzymes and proteins, are strongly regulated by small pH changes. These changes affect in turn biological activities such as enzyme catalysis, protein assembly, membrane function and cell death. There is also a strong relation between cancer and pH.
Cancer cells often display a lower pH compared to normal cells: the pH level inside cancer cells is higher than it is outside. "In living organisms, these small pH changes typically occur in tiny areas measuring only few hundred nanometers," says senior author Prof. Francesco Ricci. "Developing sensors or nanomachines that can measure pH changes at this scale should prove of utility for several applications in the fields of in-vivo imaging, clinical diagnostics and drug-delivery."
"DNA represents an ideal material to build sensors or nanomachines at the nanometer scale" says senior author Prof. Vallée-Bélisle. "By taking advantage of a specific DNA sequences that form pH-sensitive triple helix, we have designed a versatile nanosensor that can be programmed to fluoresce only at specific pH values." Fluorescence is the emission of radiation, including visible light, caused by an exchange of energy. "This programming ability represents a key feature for clinical applications – we can design a specific sensor to send a fluorescent signal only when the pH reaches a specific value which is, for example, characteristic of a specific disease," adds first author Andrea Idili.
In the future, this recently patented nanotechnology may also find applications in the development of novel drug-delivery platforms that release chemio-therapeutic drugs only in the viscinity of tumor cells.
Explore further: DNA clamp to grab cancer before it develops
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Jul 10, 2013
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Novel drug cocktail may improve clinical treatment for pancreatic cancer
May 01, 2014
Pancreatic cancer is the fourth leading cause of cancer deaths in the U.S. and has the lowest overall survival rate of all major cancers (~6%). With current treatment options being met with limited success it is anticipated ...
pH-dependent conformation change controls energy transfer in nanocrystal sensors
Dec 14, 2012
(Phys.org)—Acidity (pH) and its changes play an important role in many physiological processes, including protein folding, and can act as indicators of cancer. In the journal Angewandte Chemie, American resear ...
Researchers identify biomarker for smoker's lung cancer
Sep 19, 2013
Mayo Clinic researchers have shown that a specific protein pair may be a successful prognostic biomarker for identifying smoking-related lung cancers. The protein—ASCL1—is associated with increased expression of the RET ...
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Having eczema may reduce your risk of skin cancer
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© Medical Xpress 2011-2014, Science X network
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