Monday, 30 March 2015

Study debunks common misconception that urine is sterile





Bacteria have been discovered in the bladders of healthy women, discrediting the common belief that normal urine is sterile. This finding and its implications were addressed in an editorial published by researchers from Loyola University Chicago Stritch School of Medicine (SSOM) in the latest issue of European Urology.



"Clinicians previously equated the presence of bacteria in urine to infections. The discovery of bacteria in the urine of healthy females provides an opportunity to advance our understanding of bladder health and disease," said Alan Wolfe, PhD, lead author and professor in the Department of Microbiology & Immunology, SSOM. "Physicians and researchers must reassess their assumptions surrounding the cause of lower disorders and consider new approaches to prevent and treat these debilitating health issues."


Stritch researchers evaluated urine specimens collected directly from the bladder through an aspiration or a catheter to avoid contamination. These specimens were analyzed using an expanded quantitative urine culture (EQUC) technique that identifies bacteria not detectable by the standard urine culture techniques typically used to diagnose urinary tract syndromes. This study also used 16S rDNA sequencing to classify bacterial DNA.


"While traditional urine cultures have been the gold standard to identify disorders in the past, they do not detect most bacteria and have limited utility as a result," Dr. Wolfe said. "They are not as comprehensive as the testing techniques used in this study."


Through their analysis, Loyola researchers found that certain bacteria in the female bladder may contribute to symptoms of . They also revealed that some bacteria are more common in women with urgency urinary incontinence than in healthy women.


"If we can determine that select bacteria cause various lower urinary tract symptoms, we may be able to better identify those women at risk and more effectively treat them," said Linda Brubaker, MD, MS, co-author, dean and chief diversity officer, SSOM.


Loyola researchers will further explore the role of urinary in health and disease to learn more about common lower urinary tract disorders such as urinary tract infections, overactive bladder, urinary incontinence and painful bladder syndromes.



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Bacteria have been discovered in the bladders of healthy women, discrediting the common belief that normal urine is sterile. This finding and its implications were addressed in an editorial published by researchers from Loyola University Chicago Stritch School of Medicine (SSOM) in the latest issue of European Urology.



"Clinicians previously equated the presence of bacteria in urine to infections. The discovery of bacteria in the urine of healthy females provides an opportunity to advance our understanding of bladder health and disease," said Alan Wolfe, PhD, lead author and professor in the Department of Microbiology & Immunology, SSOM. "Physicians and researchers must reassess their assumptions surrounding the cause of lower disorders and consider new approaches to prevent and treat these debilitating health issues."


Stritch researchers evaluated urine specimens collected directly from the bladder through an aspiration or a catheter to avoid contamination. These specimens were analyzed using an expanded quantitative urine culture (EQUC) technique that identifies bacteria not detectable by the standard urine culture techniques typically used to diagnose urinary tract syndromes. This study also used 16S rDNA sequencing to classify bacterial DNA.


"While traditional urine cultures have been the gold standard to identify disorders in the past, they do not detect most bacteria and have limited utility as a result," Dr. Wolfe said. "They are not as comprehensive as the testing techniques used in this study."


Through their analysis, Loyola researchers found that certain bacteria in the female bladder may contribute to symptoms of . They also revealed that some bacteria are more common in women with urgency urinary incontinence than in healthy women.


"If we can determine that select bacteria cause various lower urinary tract symptoms, we may be able to better identify those women at risk and more effectively treat them," said Linda Brubaker, MD, MS, co-author, dean and chief diversity officer, SSOM.


Loyola researchers will further explore the role of urinary in health and disease to learn more about common lower urinary tract disorders such as urinary tract infections, overactive bladder, urinary incontinence and painful bladder syndromes.



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date Oct 17, 2013

Women with common forms of urinary incontinence have various bacteria in their bladder, according to data presented today by researchers from Loyola University Chicago Stritch School of Medicine. Researchers also found that ...



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date May 18, 2014

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Study debunks common myth that urine is sterile


date Apr 09, 2012

Researchers have determined that bacteria are present in the bladders of some healthy women, which discredits the common belief that normal urine is sterile. These findings were published in the April issue of the Journal of ...



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date Dec 11, 2014

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